Animal Cell Technology: Basic & Applied Aspects: Proceedings by K. Koshimizu (auth.), S. Kaminogawa, A. Ametani, S.

By K. Koshimizu (auth.), S. Kaminogawa, A. Ametani, S. Hachimura (eds.)

Animal telephone know-how has been making super growth. initially this time period reminded humans of engineering for top density and massive quantity tradition of animal cells. at this time many fields of organic sciences are aiming at enhance in animal mobilephone expertise. mobilephone tradition engineering is aided not just with advancements in gear, matrix, media, and computational research, but additionally with new organic approaches in gene and protein expertise, mobilephone organic assets and immunological tools. effects bought with animal cellphone know-how are utilized to construction of prescription drugs, prognosis reagents and nutrition endowed with physiological services, and cellphone and gene remedy of animals and people, and helpful for elucidating clinical phenomena. it's also necessary to identify equipment of review for performance and safeguard of newly chanced on molecules and cells. The development in animal mobile know-how is supported by means of, and attributes in either one of uncomplicated and technologies. The lawsuits of the 5th foreign assembly of the japanese organization for Animal phone know-how (JAACT) covers the topics above pointed out. The articles during this booklet can assist researchers in lots of fields to appreciate the present prestige and destiny tendencies in animal mobilephone know-how. JAACT prepared this assembly and we show our gratitude to the individuals of JAACT. We gratefully recognize all of the individuals of the organizing committee for his or her commitment in assuring the Meeting's luck. for his or her helpful helps, we additionally thank the japanese Biolndustry organization and Saitama starting place for tradition and Industry.

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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Fifth International Meeting of the Japanese Association for Animal Cell Technology, Omiya, Japan, November 30—December 4, 1992

Example text

After reculturing in the serum-free culture medium, the cells were injected subcutaneously into the back of Balb/c mice. All of the mice which had received ras/myc SFME Cell +--- pSV2neo ~ G418 resistant Cell ~ Balb/c Subcutaneous 1[ ~ Mouse with MetastaUe Tumors Lymph Nods Lung Li ver Lung ~ 1 Balb/c Subcutaneous --+Tumor (Seven tim~s Transfers) t L ln Vitro ~ Lung Culture (SF) [ Plate 1] Metastalle Tumors in Lung at 3rd Transfer 22 each of four independently transfected cells developed solid tumors at the injected sites within 2 months.

2. MTT assay Cells were cultured in 96-well plates. A 5 mg/ml MTT/PBS solution was added to each weil, and the plates were cultured for 6 hours. l/well), and the absorbance at 570 nm was measured. 3. Measurement of ALP activity Cells were washed with PBS, before the ALP activity and protein content were measured. 25 mg/ml p-nitrophenyl phosphate solution in a 1 M diethanolamine buffer was added before incubating for 30 min at 37°C. l of 1 N NaOH to the wells. The absorbance at 405 nm was measured, and the concentration of the product was determined against a standard curve drawn for p-nitrophenol.

And Trier, J. S. (1987) in L. R. Johnson (ed), Physiology of the Gastrointestinal Tract, Raven Press, New York, pp. 1209-1250. 2. Moog, F. (1979) in K. Elliot and J. Whelan (eds), Development of Mammalian Absorptive Process (Ciba Foundation Symposiun 70), Exerpta Medica, Amsterdam, pp. 31-44. 3. , Appay, M. , and Zweibaum A. (1982) Biol. Cell. 44, 193-196. 4. , and Rousset, M. (1985) J. Cell Physiol. 122, 21-29. 5. Wice, B. , Lacroix, B. and Zweibaum, A. (1985) J. Biol. , 260, 139-146. 6. , Brun, J.

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